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Fungal diversity in soil and durum wheat was studied in relation to durum, oilseed and pulse preceding crops, durum growth stages and plant organs. Fungi isolated from soil and plant samples were classified in 258 operational taxonomical units (OTUs) followed by the PCR amplification and sequencing of the internal transcribed spacer (ITS) rDNA and elongation factor EF-1 alpha gene. Most of the 46...
Pyrenophora species, toxigenic cereal pathogens, and causal agents of leaf and kernel diseases, bring about economic and food safety concerns. Traditionally, Pyrenophora taxa have been identified microscopically after a period of incubation on culture media. In this study, a simple nested PCR–denaturing gel electrophoresis (DGGE) method was developed to detect, differentiate and identify six Pyrenophora...
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