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Ferredoxin: glutamate synthase from spinach and soybean chloroplasts has been recognized as a new member of thioredoxin-dependent enzymes in higher plants. For this purpose, a new ferredoxin: glutamate synthase assay procedure had to be established. The assay mixture was filtered through size exclusion filter units to separate the low molecular substrates and products from the proteins that stop the...
Glutaredoxin(thioltransferase) has been identified and purified to homogeneity from spinach leaves. Its cytosolic localization was demonstrated by chromatographic and immunological analysis of extracts from isolated spinach chloroplasts and mitochondria, respectively. Spinach glutaredoxin shows a significant crossreactivity with antibodies raised against E. coli glutaredoxin and possesses a specific...
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