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Cytosolic glutathione S-transferase activity was studied in the three developmental stages of Tenebrio molitor. The highest activity towards both CDNB and DCNB was observed in the newly emerged pupae, while towards ethacrynic acid was in the young larvae. The pH optimum of GST activity towards CDNB was 8.5 in larvae and pupae and 8.25 in adults. GSTs operate at a high rate even at pH values 1 or 2...