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Main conclusionThe bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50% D2O to obtain biomass with 34% deuterium substitution and physicochemical properties similar to those of H2O-grown switchgrass controls. Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate...
To further investigate the effect of dilute acid pretreatment (DAP) and steam explosion pretreatment (SE) on the change in cellulose accessibility, several techniques were applied including a Simons’ stain (SS) technique along with several NMR methods (i.e., NMR cryoporometry, 1H spin–lattice (T1) and 1H spin–spin (T2) relaxometry, and diffusometry). These methods were utilized to probe biomass porosity...
Perdeuterated pyridinium chloride–DMSO-d 6 is an effective solvent system for whole cell biomass dissolution and NMR characterization. Employing this solvent system, semi-quantitative 13 C– 1 H heteronuclear single quantum correlation (HSQC) spectroscopy of untreated, steam, dilute acid and lime pretreated poplar biomass samples was readily accomplished. In an effort to demonstrate...
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