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We report on the implementation of a fully aseptic, single‐use ReadyToProcess™ cross flow filtration system for scalable clarification and concentration of a cytomegalovirus‐like particle (CMV reVLP®), in preclinical development at Redbiotec AG, using ultrafiltration/diafiltration (UF/DF). Performed under gentle feed and permeate flow conditions, with a transmembrane pressure below 0.8 bar and a flux...
We recently described the efficient assembly of hepatitis C virus (HCV) structural proteins into HCV-like particles (HCV-LPs) in insect cells. These noninfectious HCV-LPs have similar morphologic and biophysical properties as putative virions isolated from HCV-infected humans and can induce a broadly directed immune response in animal models. The HCV envelope proteins of HCV-LPs are presumably presented...
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