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This paper reports a novel expression system constructed to maximize the production inEscherichia coliofd-amino acid oxidase from the yeastRhodotorula gracilis(RgDAAO). We produced a recombinant plasmid by the insertion of the cDNA encoding for the RgDAAO into the multiple cloning site of the expression vector pT7.7 (pT7-DAAO), downstream of the T7 RNA polymerase binding site. The pT7-DAAO, which...