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Citrate synthase has been purified to homogeneity from the thermophilic archaebacteria Thermoplasma acidophilum and Sulfolobus acidocaldarius. From the relative molecular masses of the native proteins (85 and 83 kDa, respectively) and of their polypeptide chains (43 and 41 kDa, respectively) it is established that they are dimeric enzymes. The N-terminal sequence of the Thermoplasma citrate synthase...