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The adipose stromal vascular fraction (SVF) contains abundant mesenchymal stem cell populations that have a limited ability to self‐renew and differentiate. Male mouse adipose SVF cells were dedifferentiated by reprogramming factors (c‐Myc, Oct4, Sox2, and Klf4) to form embryonic stem cell‐like cells (ESCLCs), which upgraded their limited differentiation potential. The ESCLCs were induced to differentiate...
Mouse embryonic fibroblasts (MEFs) have been used as feeder cells to support the growth of mouse embryonic stem cell (mESC) and primordial germ cells (PGC) in culture for many years. However, MEF preparation is a complex and tedious task. Recently, there are reports indicating that the microenvironment provided by bone marrow stromal cells could support the survival of embryonic-like stem cells in...
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