Search results for: Cristiana Campa

Items from 1 to 20 out of 22 results

article

Quality by design approach in the development of an ultra-high-performance liquid chromatography method for Bexsero meningococcal group B vaccine

Luca Nompari, Serena Orlandini, Benedetta Pasquini, Cristiana Campa, more

Talanta > 2018 > 178 > C > 552-562

Bexsero is the first approved vaccine for active immunization of individuals from 2 months of age and older to prevent invasive disease caused by Neisseria meningitidis serogroup B. The active components of the vaccine are Neisseria Heparin Binding Antigen, factor H binding protein, Neisseria adhesin A, produced in Escherichia coli cells by recombinant DNA technology, and Outer Membrane Vesicles (expressing...

chapter

Capillary Electrophoresis for Monitoring Natural and Synthetic Processes of Saccharide-Bearing Molecules

Marco Rossi, Amelia Gamini, Cristiana Campa

Capillary Electrophoresis of Carbohydrates > 151-172

A survey of the more recent applications and developments of capillary electrophoresis (CE) to study reactions involving saccharide-bearing molecules is presented. Selected studies are cited to highlight the potentialities as well as the versatility of capillary electrophoresis. CE strategies that are applied to monitoring the synthesis of carbohydrate-based molecules are discussed. One strategy is...

article

Borate complexes of X-ray iodinated contrast agents: Characterization and sorption studies for their removal from aqueous media

Isabella Rustighi, Ivan Donati, Matteo Ferluga, Cristiana Campa, more

Journal of Hazardous Materials > 2012 > 205-206 > Complete > 10-16

Iodinated contrast media (ICM) are persistent and ubiquitous water pollutants. Because of their high water solubility and biochemical stability, their phase-separation and recovery from the aquatic environment is very difficult. Here, borate was chosen as a complexing agent of the two diagnostic aids iomeprol and iopamidol in order to provide them with a negative charge and to fix the resulting adducts...

article

Novel fluorescent cycloheximide derivatives for the imaging of protein synthesis

Francesca Paoletti, Kevin Ainger, Ivan Donati, Raffaella Scardigli, more

Biochemical and Biophysical Research Communications > 2010 > 396 > 2 > 258-264

Cycloheximide (CHX) is one of the most interesting protein synthesis inhibitors. For this reason, fluorescent derivatives of CHX could find useful applications in cell biology. We report the successful synthesis of a set of novel fluorescent derivatives of CHX. The effect of different functional groups on the biological activity of CHX was studied upon their modification through suitable strategies,...

article

Structural organization of NadAΔ351–405, a recombinant MenB vaccine component, by its physico-chemical characterization at drug substance level

Claudia Magagnoli, Angela Bardotti, Giuseppe De Conciliis, Rosy Galasso, more

Vaccine > 2009 > 27 > 15 > 2156-2170

The physico-chemical characterization of NadAΔ _351–405 , a recombinant protein discovered by reverse vaccinology, component of a candidate vaccine against Neisseria meningitidis serotype B is presented. Analytical methods like mass spectrometry, electrophoresis, optical spectroscopy and SEC-MALLS have been applied to unveil the structure of NadAΔ _351–405 , and to evaluate Product-Related...

article

X-ray studies on ternary complexes of maltodextrin phosphorylase

Mara Campagnolo, Cristiana Campa, Rita De Zorzi, Jochen Wuerges, more

Archives of Biochemistry and Biophysics > 2008 > 471 > 1 > 11-19

We report crystal structures of ternary complexes of maltodextrin phosphorylase with natural oligosaccharide and phosphate mimicking anions: nitrate, sulphate and vanadate. Electron density maps obtained from crystals grown in presence of Al(NO ₃ ) ₃ show a nitrate ion instead of the expected AlF4- in the catalytic site. The trigonal NO3- is coplanar with the Arg569 guanidinium group...

article

Study of molar response of dextrans in electrochemical detection

Franco Abballe, Mila Toppazzini, Cristiana Campa, Fulvio Uggeri, more

Journal of Chromatography A > 2007 > 1149 > 1 > 38-45

In this work, a methodological approach is reported, aimed at assessing the electrochemical response of some model gluco-oligosaccharides (dextrans). Such strategy is based on the complementary use of both anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD) and capillary zone electrophoresis coupled with UV detection (CZE–UV). Unlike HPAEC–PAD, CZE–UV required derivatization...

article

Enzymatic synthesis of the Tn antigen

Anna Coslovi, Cristiana Campa, Anna Flamigni, Marco Rossi, more

Journal of Molecular Catalysis. B, Enzymatic > 2007 > 45 > 3-4 > 128-134

The enzymatic synthesis of the Tn antigen (GalNAc-α-O-Ser), a glyco-aminoacid of great biological importance, is reported. The reaction was promoted by commercial α-N-acetylgalactosaminidase from Acremonium sp., using p-nitrophenyl-α-N-acetylgalactosamine as the donor. The kinetics were monitored by capillary electrophoresis and LC–UV-MS. For unprotected serine, the role of pH and temperature was...

article

Separation of O- and C-allyl glycoside anomeric mixtures by capillary electrophoresis and high-performance liquid chromatography

Marco Rossi, Cristiana Campa, Amelia Gamini, Anna Coslovi, more

Journal of Chromatography A > 2006 > 1110 > 1-2 > 125-132

Rapid and reliable methods for the analysis of O- and C-allyl galactopyranosides and glucopyranosides are presented, based on capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC). In MEKC, the formation of chromophoric and charged complexes between the saccharides and borate as well as the hydrophobic interactions with micelles jointly contributed to the...

article

The role of Galectin-1 in the interaction between chondrocytes and a lactose-modified chitosan

Patrizia Marcon, Eleonora Marsich, Amedeo Vetere, Pamela Mozetic, more

Biomaterials > 2005 > 26 > 24 > 4975-4984

Evidences for the involvement of the Galectin-1 in the interaction of pig chondrocytes with a lactose-modified chitosan, namely Chitlac, are reported. The Chitlac glycopolymer has been shown to promote pig chondrocyte aggregation and to induce extracellular matrix production. Highly pure Galectin-1 was obtained from pig spleen by affinity chromatography and its identity was determined by ion spray...

article

Determination of average degree of polymerisation and distribution of oligosaccharides in a partially acid-hydrolysed homopolysaccharide: A comparison of four experimental methods applied to mannuronan

Cristiana Campa, Astrid Oust, Gudmund Skjåk-Bræk, Berit Smestad Paulsen, more

Journal of Chromatography A > 2004 > 1026 > 1-2 > 271-281

The average degree of polymerisation (DP) and distribution of oligosaccharides in partially acid hydrolysed mannuronans were quantitatively evaluated by ¹ H NMR, electrospray ionisation mass spectrometry (ESI-MS), micellar electrokinetic capillary chromatography with UV detection (MEKC-UV), and high-pressure anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Our...

article

Galactose-Substituted Alginate 2: Conformational Aspects

Ivan Donati, Anna Coslovi, Amelia Gamini, Amedeo Vetere, more

Biomacromolecules > 2004 > 5 > 1 > 186 - 196

article

Galactose-Substituted Alginate: Preliminary Characterization and Study of Gelling Properties

Ivan Donati, Amedeo Vetere, Amelia Gamini, Anna Coslovi, more

Biomacromolecules > 2003 > 4 > 3 > 624 - 631

article

Determination of the diadic composition of alginate by means of circular dichroism: a fast and accurate improved method

Ivan Donati, Amelia Gamini, Gudmund Skjåk-Bræk, Amedeo Vetere, more

Carbohydrate Research > 2003 > 338 > 10 > 1139 - 1142

Circular dichroism (CD) is presented as a reliable and sensitive method of determining the diadic frequency composition of alginate (F _G _G , F _M _M and F _G _M ₊ _M _G ). The availability of samples, very largely or even completely conforming to the limiting structures of polymannuronate (MM) _n , polyguluronate (GG) _n and polyalternating MG...

article

High-yield enzymatic synthesis of O-allyl β-d-galactopyranoside

Amedeo Vetere, Michela Medeot, Cristiana Campa, Ivan Donati, more

Journal of Molecular Catalysis. B, Enzymatic > 2003 > 21 > 3 > 153-156

A high-yield synthesis of O-allyl β-d-galactopyranoside was carried out by the use of Aspergillus oryzae β-galactosidase. The reaction was carried out employing p-nitrophenyl β-d-galactopyranoside as the donor and a large excess of allyl alcohol as the acceptor. The molar yield was 65.6%, corresponding to an improvement of 41.3% with respect to the best results previously reported with other systems,...

article

Structure of the oligomers obtained by enzymatic hydrolysis of the glucomannan produced by the plant Amorphophallus konjac

Paola Cescutti, Cristiana Campa, Franco Delben, Roberto Rizzo

Carbohydrate Research > 2002 > 337 > 24 > 2505 - 2511

Dimers and trimers obtained by enzymatic hydrolysis of the glucomannan produced by the plant Amorphophallus konjac were analysed in order to obtain information on the saccharidic sequences present in the polymer. The polysaccharide was digested with cellulase and β-mannanase and the oligomers produced were isolated by means of size-exclusion chromatography. They were structurally characterised using...

article

Enzymatic synthesis and characterization of oligosaccharides structurally related to the repeating unit of Pullulan

Cristiana Campa, Amedeo Vetere, Amelia Gamini, Ivan Donati, more

Biochemical and Biophysical Research Communications > 2002 > 297 > 2 > 382-389

A trisaccharide (Glcα1-4Glcα1-6Glc) and a tetrasaccharide (Glcα1-4Glcα1-4Glcα1-6Glc) the structures of which are related to that of repeating unit of pullulan have been obtained, exploiting the transglycolytic activity of Aspergillus niger cyclodextrin glucanotransferase. Both products were obtained in one-pot reaction using as a donor the α-cyclodextrin and as an acceptor the disaccharide isomaltose...

article

Synthesis, Characterization, and Preliminary Biological Study of Glycoconjugates of Poly(styrene- co -maleic acid)

Ivan Donati, Amelia Gamini, Amedeo Vetere, Cristiana Campa, more

Biomacromolecules > 2002 > 3 > 4 > 805 - 812

article

Regiospecific Transglycolytic Synthesis and Structural Characterization of 6-O-α-Glucopyranosyl– Glucopyranose (Isomaltose)

Amedeo Vetere, Amelia Gamini, Cristiana Campa, Sergio Paoletti

Biochemical and Biophysical Research Communications > 2000 > 274 > 1 > 99-104

The enzymatic synthesis of 6-O-α-glucopyranosyl–glucopyranose (isomaltose) was achieved. The regiospecific transglycosylation reaction was catalyzed by a crude preparation of α-d-glucosidase from Aspergillus niger, using p-nitrophenyl α-d-glucopyranose as the donor and glucopyranose as the acceptor. The yield of the reaction was 59% on a molar basis with respect to the donor. The structural identity...

article

Isocratic separations of closely-related mono- and disaccharides by high-performance anion-exchange chromatography with pulsed amperometric detection using dilute alkaline spiked with barium acetate

Tommaso R.I Cataldi, Cristiana Campa, Massimiliano Angelotti, Sabino A. Bufo

Journal of Chromatography A > 1999 > 855 > 2 > 539-550

Mixtures of closely related mono- and disaccharides may be efficiently separated by high-performance anion-exchange chromatography (HPAEC) only when relatively dilute alkaline eluents are employed (i.e., <20 mM NaOH). The main drawbacks of these eluent solutions are (i) column regeneration between runs, (ii) poor reproducibility of the retention times, and (iii) the need for post-column base addition...

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Search results for: Cristiana Campa

Quality by design approach in the development of an ultra-high-performance liquid chromatography method for Bexsero meningococcal group B vaccine

Capillary Electrophoresis for Monitoring Natural and Synthetic Processes of Saccharide-Bearing Molecules

Borate complexes of X-ray iodinated contrast agents: Characterization and sorption studies for their removal from aqueous media

Novel fluorescent cycloheximide derivatives for the imaging of protein synthesis

Structural organization of NadAΔ351–405, a recombinant MenB vaccine component, by its physico-chemical characterization at drug substance level

X-ray studies on ternary complexes of maltodextrin phosphorylase

Study of molar response of dextrans in electrochemical detection

Enzymatic synthesis of the Tn antigen

Separation of O- and C-allyl glycoside anomeric mixtures by capillary electrophoresis and high-performance liquid chromatography

The role of Galectin-1 in the interaction between chondrocytes and a lactose-modified chitosan

Determination of average degree of polymerisation and distribution of oligosaccharides in a partially acid-hydrolysed homopolysaccharide: A comparison of four experimental methods applied to mannuronan

Galactose-Substituted Alginate 2: Conformational Aspects

Galactose-Substituted Alginate: Preliminary Characterization and Study of Gelling Properties

Determination of the diadic composition of alginate by means of circular dichroism: a fast and accurate improved method

High-yield enzymatic synthesis of O-allyl β-d-galactopyranoside

Structure of the oligomers obtained by enzymatic hydrolysis of the glucomannan produced by the plant Amorphophallus konjac

Enzymatic synthesis and characterization of oligosaccharides structurally related to the repeating unit of Pullulan

Synthesis, Characterization, and Preliminary Biological Study of Glycoconjugates of Poly(styrene- co -maleic acid)

Regiospecific Transglycolytic Synthesis and Structural Characterization of 6-O-α-Glucopyranosyl– Glucopyranose (Isomaltose)

Isocratic separations of closely-related mono- and disaccharides by high-performance anion-exchange chromatography with pulsed amperometric detection using dilute alkaline spiked with barium acetate

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