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Our focus was to develop a three-dimensional (3D) human dynamic in vitro tissue model that mimics the natural microenvironment of the small intestine. We co-cultured human Caco-2 cells with primary-isolated human microvascular endothelial cells (hMECs) on decellularized porcine jejunal segments within a custom-made dynamic bioreactor system that resembles the apical and basolateral side of the intestine...
Tissue engineering represents a biology driven approach by which bioartificial tissues are engineered through combining material technology and biotechnology. In order to ensure the functionality of in vitro cultured cells, culture conditions simulating the natural microenvironment must be created. Therefore (1) sufficient nutrient supply of the cells, (2) co-culture of different cell types, (3) suitable...
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