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The “marcha” is a comfortable equestrian four-beat gait characterized as a submaximal exercise. Four healthy Mangalarga Marchador horses of 6–7 years of age were assessed under field conditions covering distances of 12.7, 19.3, and 38.8 km. Blood samples were collected before, during, and at the end of the exercise, as well as after 4 hours of rest, to measure the hematological, biochemical, and enzymatic...