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In the present study we have studied the changes in the intracellular reduction-oxidation state in mouse pancreatic acinar cells following stimulation with cholecystokinin octapeptide (CCK-8) and its dependence on Ca 2+ mobilization. In our investigations cytosolic Ca 2+ concentration and reactive oxygen species (ROS) production were determined by loading of cells with fura-2...
In the present study, we have employed confocal laser scanning microscopy to investigate the effect that stimulation of mouse pancreatic acinar cells with the secretagogue cholecystokinin (CCK) has on mitochondrial activity. We have monitored changes in cytosolic as well as mitochondrial Ca 2+ concentrations, mitochondrial membrane potential and FAD autofluorescence by loading the cells...
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