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A transient transfection system has been developed for a member of the Apicomplexa, Eimeria tenella, using β-galactosidase (βgal) from Escherichia coli as the reporter enzyme. Successfully expressed constructs contained sequences of the E. tenella microneme gene Etmic-1 fused to the coding region of lacZ. Transfectants expressing βgal were able to invade host cells and proceed through part of the...