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Kidney acetone powders (KAP) from beef, dog, hog, rat, and sheep have been evaluated for resolving N-acetyl-dl-homophenylalanine. We also propose a simple protocol for the preparation of both enantiomers of homophenylalanine by enzymatic resolution using mammalian KAP. The beef kidney afforded the best results, rendering the highest isolated yields, 37.5% and 41% and enantiomeric excesses of 94% and...
An easy, inexpensive and accurate method to determine enantiomeric excess of cyanohydrins is described. The method consists in derivatization of cyanohydrins with (S)-naproxen® chloride and the products analyzed by HPLC. The results of enantiomeric excess so determined are similar to those obtained with [Eu(hfc) 3 ], as a chiral shift reagent, and analysis by 1 H-NMR of the cyanohydrins.
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