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We constructed one wild-type xylose reductase (XR) and eight XR variants in a recombinant industrial Saccharomyces cerevisiae strain along with xylitol dehydrogenase and xylulokinase overexpression. In the current study, we found that K270R XR (hereafter referred to as “XR-1”) appeared to utilize more NADH than NADPH when xylose was the carbon source. After 72h of fermentation, XR-1 produced an ethanol...