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A new voltammetric method for a direct determination of gold nanoparticles, based on adsorption and electrochemical detection of colloidal gold, is described. In this protocol, the absorption of gold nanoparticles onto the rough surface of graphite-epoxy composite electrode is followed by their electrochemical oxidation in 0.1M HCl medium at a potential of +1.25V. The resulting tetrachloroaurate ions...
We have developed a procedure for the accurate measurement of histidine decarboxylase in tissues expressing low levels of enzymatic activity. Briefly, histamine is enzymatically synthesized from [ 3 H]-labeled histidine, followed by purification using high-performance liquid chromatography (HPLC) and quantitation by liquid scintillation counting. This method presents three advantages over...
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