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We introduce stimulated Raman scattering microscopy which allows us to visualize unstained live cells with high sensitivity and high contrast. The shot-noise limited sensitivity can be achieved based on high-frequency lock-in detection using subharmonically synchronized two-color pulses.
We propose and experimentally show that stimulated Raman scattering can realize novel 3D nonlinear-optical Raman microscopy that is immune to nonresonant background signals. We demonstrate high-contrast molecular imaging of polystyrene beads and plant cells.
When ultrafast laser pulses are tightly focused inside a transparent medium, various nonlinear optical phenomena readily occur at the focal point. These phenomena at the focal point can be utilized for nonlinear-optical microscopy, nanosurgery inside biological samples, device fabrication inside transparent materials, and welding of transparent materials. We review these new techniques from a unified...
The basics of three-dimensional (3-D) and spectral imaging techniques that are based on the detection of coherence functions and other related techniques are reviewed. The principle of the 3-D source retrieval is based on understanding the propagation law of optical random field through the free space. The 3-D and spectral information are retrieved from the cross-spectral density function of optical...
We propose a novel technique of four-wave mixing (FWM) microspectroscopy enhanced by a two-photon electronic resonance of pump pulses along with the stimulated emission induced by a dump pulse
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