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Background Adipose-tissue stem cells (ASCs) are subject of intensive research since their successful use in regenerative therapy. The drawback of ASCs is that they may serve as stroma for cancer cells and assist tumor progression. It is disquieting that ASCs frequently undergo genetic and epigenetic changes during their in vitro propagation. In this study, we describe the polyploidization of murine...
Direct reprogramming of mouse fibroblasts into induced pluripotent stem cells (iPS) was achieved recently by overexpression of four transcription factors encoded by retroviral vectors. Most of the virus vectors, however, may cause insertional mutagenesis in the host genome and may also induce tumor formation. Therefore, it is very important to discover novel and safer, non-viral reprogramming methods...
Villa lo Zerbino, built at the end of the 16th century, is an example of an Alessian villa in a suburban area of Genoa. During the centuries, the expanding city reached the villa, later incorporating it completely. As the surrounding urban landscape evolved, the garden was also modified, following the English landscape trends of the era. The main objective of the project discussed in this paper is...
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