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Recent studies have shown that stimulation of osteoclastogenesis in cocultures of osteoblasts and spleen cells in response to prostaglandin E 2 (PGE 2 ) is markedly decreased when the osteoblasts are derived from cells lacking either the EP 2 or the EP 4 receptor. Induction of osteoclast formation requires upregulation of receptor activator of nuclear factor-κB ligand...
To study the anabolic effects of prostaglandins (PGs) on osteoblastic differentiation, we developed a model using primary cultures of mouse bone marrow cells. 5 to 7 week old CD-1 mice were sacrificed and the bone marrow flushed out from the femur and tibia. Unfractionated marrow cells were plated at high density (1.5 - 3.5 10 6 cells/ cm 2 ) in DMEM containing 10% fetal bovine...
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