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Protein synthesis directed by hepatitis A virus (HAV) RNA is mediated by a mechanism involving the recognition of internal sequences. Two in-frame AUG codons initiate the long open reading frame (positions 734-736 and 740-742). The extra-cistronic region extending between the uncapped 5'-end and the ORF contains two pyrimidine-rich tracts (PRTs): one 12 nucleotides in length in the close vicinity...
The 5 -terminal untranslated region (5 -UTR) of picornavirus RNA contains a series ofcis -acting elements required for the internal initiation of translation, including a pyrimidine-rich tract (PRT), which in entero- and rhinoviruses is located about 20 nts upstream from a silent AUG triplet in the vicinity of the translation initiation site. In hepatitis A virus (HAV) RNA, the PRT is only 12 nts...
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