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The aim of this study was to evaluate the acute phase response of dairy cows to repeated liver biopsy in order to estimate the safety of this procedure during the transition period. Liver biopsies (up to 1000 mg of liver tissue) were conducted twice a day, 7 days before expected parturition and 3 days after calving. The number of needle insertions for each biopsy was recorded and was dependent on...
Celem pracy było porównanie kilku metod szacowania dobowej wydajności mleka na podstawie jednego udoju: porannego lub wieczornego, krów ocenianych metodą AT4. Materiał stanowiły dane z robotów udojowych udostępnione przez Polską Federację Hodowców Bydła i Producentów Mleka (PFHBiPM). Wybrano 43 309 udojów wykonanych między 5. a 305. dniem doju dla krów z jednym lub dwoma udojami w ciągu doby. Odstęp...
Measuring gene expression is a commonly used method to monitor the reaction of cells and tissues to changing nutritional or physiological conditions. Selection of appropriate reference genes is a crucial point in gene expression experiments using real-time PCR techniques. Expression of the “ideal” reference gene should not be affected by the experimental treatments or physiological state of the tissue,...
The effect of the transition of dairy cows from pregnancy to lactation on the expression of genes encoding enzymes involved in hepatic glucose metabolism was studied. Six Holstein-Friesian heifers were used for this study. Liver samples were collected by biopsy on day 7 before expected parturition (-7) and days 3 and 21 after parturition (+3 and +21, respectively). The mRNA levels of pyruvate carboxylase(PC),phosphoenolpyruvate...
One of the largest challenges for the dairy industry is to provide cows with a diet which is highly energetic but does not negatively affect their rumens’ functions. In highly productive dairy cows, feeding diets rich in readily fermentable carbohydrates provides energy precursors needed for maximum milk production, but simultaneously decreases ruminal pH, leading to a widespread prevalence of subacute...
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