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The aim of the study was to apply the real-time PCR method with the intercalating dye SYBR Green I for the detection of genes of Bacillus anthracis located on plasmids pXO1 and pXO2 and the specific chromosomal rpoB sequence. The research was conducted on one vaccinal and four field strains of Bacillus anthracis. The assessment of the specificity of the tests involved isolates of other species of...
Evaluation of the immunogenicity of the vaccine against respiratory tract infections in calves caused by Pasteurella multocida, and prepared from antigen of a local strain of serotype 3 with oil adjuvant addition, was the object of the prescnt studies. Two experiments were carried out using calves. Vaccine was given them at a dose of 2 ml i.m., twice every 14 days. In experiment 1, sera were obtained...
The goal of the experiment was to evaluate the Boviseptivac vaccine against cattle pasteurellosis manufactured from local isolates of P. multocida serotype B:2 with the addition of an oil adjuvant (Emulsigen). Field examinations were performed on 645 cows in the Ostroleka province. The vaccine effectively protected against the disease; however, 6 (93%) of examined cows revealed shock signs which were...
Investigations aimed to evaluate the effectiveness of Boviseptivac, vaccine against bovine pasteurellosis prepared from the Polish isolates of serotype B:2 with an oil adjuvant (Emulsigen). The vaccine protective value obtained on white mice was 4.25 according to the method of Ose and Muenster. Field trials carried out on 120 cows in the Ostroleka province revealed advantageous properties of the vaccine,...
The ELISA was used to measure the level of specific lgG to P. multocida in the sera of rabbits vaccinated against pasteurellosis on a farm at Ch. The vaccines comprised the antigens of serotypes 3 and 12 with two adjuvants: emulsigen or aluminium hydroxide gel. Eight-week-old rabbits were injected subcutaneously with a 1-ml dose. Animals were observed for about 2 months until slaughter. A statistically...
The aim of the experiment was to determine, using the ELISA and gel diffusion precipitin test, the antibody serum level in rabbits immunised with vaccines comprising P.multocida antigens of serotypes 3 and 12 with two adjuvants: oil (Cunipastivac E) and aluminium hydroxide gel (Cunipastivac A). The ELISA revealed an increase in specific lgG levels against P. multocida and the positive precipitin reaction...
The goal of the studies was to evaluate the protective value of vaccines against pasteurellosis in rabbits. The vaccines comprised antigens to serotypes 3 and 12 of Pasteurella multocida with two different adjuvants: emulsigen and aluminium hydroxide gel. The two vaccines given subcutaneusly either once or twice at a dose of 1 ml protected rabbits markedly against intranasal infection with the virulent...
The goal of the study was to evaluate under field conditions the protective value of the vaccines comprising antigens to serotypes 3 and 12 of P. multocida with emulsigen (Cunipastivac E) and aluminium hydroxide gel (Cunipastivac A) against pasteurellosis in rabbits. Both vaccines were given subcutaneously at a dose of 1 ml. This study revealed: 1) No harmful effects of these vaccines and a beneficial...
The aim of this study was to determine the effect of infection with hog cholera virus on the bactericidal action of swine serum against strains of E. coli. This study also was intended to broaden the present conceptions of the mechanism of bacterial complications in the course of a virus infection (6), which is in turn connected with the problem of a quantitative evaluation of the level of natural...
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