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Dendritic cells (DC) generated from human umbilical cord blood might replace patients' DC in attempts to elicit tumor-specific immune response in cancer patients. We studied the efficiency of transfection of human cord blood DC with plasmid DNA carrying the enhanced version of green fluorescent protein (EGFP) as a reporter gene, to test if nonviral gene transfer would be a method to load DC with protein...
The aim of this study was to demonstrate the bioequivalence of two formulations base on enrofloxacin i.e. between a test formulation Enrofloksacyna 10% per os (Biowet Drwalew) and the reference Baytril 10% (Bayer), according to a framwork required for the registration files. Both formulations were administered in a single oral administration at the recommended dose of 10 mg enrofloxacin per kg of...
A simple and sensitive method for the determination of ß-agonists in animal tissue and urine using thin layer and liquid chromatography was prepared. The development of the method involves optimization of sample clean-up (solid-phase extraction) and chromatographic separation and detection conditions. The recovery of salbutamol and clenbuterol from tissue and urine samples were above 80% and the limit...
A simple and rapid method is described which allows analysis of Carazolol (ß-blocker agent) in tissue of pig by thin layer and liquid chromatography. The method utilizes an octadecyl solid phase column that selectively absorbs this drug and significantly improves sample clean-up procedure. The compound is eluted from the column with acidic acetonitrile. Analysis is performed using TLC for detection...
This survey provides a general discussion on drug residue analysis in biological material. Quality criteria for the use of thin layer chromatography in residue analysis have been evaluated and compared with those of other methods. The present application of TLC in residue analysis of antibacterials, growth promoters and neuroleptics has been discussed.
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