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The aim of the present study was to identify the deletion/insertion polymorphism of the bovine prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3' untranslated region (14 bp). DNA was isolated from blood of 234 randomly tested Polish Holstein-Friesian cows and from semen of 47 sires used for artificial insemination (AI) in 2004. No statistically significant differences...
The aim of the present study was to verify the hypothesis that α-lactalbumin (LALBA) gene polymorphism may be a factor differentiating defence mechanisms in cattle. The association between LALBA polymorphism and the levels of blood indices characterising defence functions were examined in a group of 129 female calves and young heifers, produced by random mating of parental couples. The levels of blood...
PCR modification was performed by using two sets of primers specific for BLV gag gene and bovine kappa-casein gene (CASK) which show the possibility of disclosing BLV misdiagnosis. The results obtained indicate that the described PCR modification can be proposed as a simple and reliable method of BLV provirus detection.
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