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The effects of aflatoxin B₁ (AFB₁), ochratoxin A (OTA), patulin (PAT), citrinin (CIT), and zearalenone (ZEA) on in vitro response of pig peripheral blood mononuclear cells to mitogen concanavalin A was assayed after three days of incubation using ³H- thymidine uptake. Dose response curves for each mycotoxin were generated and the concentrations producing 50% inhibition of cell proliferation (IC₅₀)...
In the presented study, ochratoxin A (OTA), patulin (PAT), and citrinit (CIT), three prevalent mycotoxins of Penicillium species, were evaluated for their response of bovine peripheral blood mononuclear cells (PBMC) stimulated in vitro by the mitogens: phytohaemagglutinin (PHA), concanavalin A (Con A), and pokeweed mitogen (PWM). The cultures of PBMC were assayed after 3-day incubation using ³H-thymidine...
Several components of sheep blood extensively catabolize nucleosides, including thymidine. Part of the radioactivity from tritiated thymidine, in whole blood cultures, is quickly rendered unincorporable as the compound is metabolized to thymine and further breakdown products. Thus, cells continue to proliferate without incorporating radioactivity from the medium. Furthemore, variability in the degree...
Decontamination effectiveness of ammonium-ferric(lll)-hexacyanoferrate(II) (AFCF) in reducing the radiocaesium transfer into dairy goat milk was examined. The most effective reduction of radioactivity burden in milk was found in animals treated with AFCF as early as the first day of radionuclide contamination. Milk radioactivity concentrations in goats treated simultaneously with ¹³⁷CsCI and hexacyanoferrate...
The kinetics of radioruthenium in rats after repeated contamination with small doses was investigated. Ruthenium-106 was poorly absorbed and rapidly eliminated from the organs and tissues of rats. No specific affinity of Ru-106 for any organ or tissue was observed except for the kidneys, where marked tendency for cumulating was found. It seems that radioruthenium is of less importance for the human...
The effect of a diet supplemented with copper (50 ppm) on the uptake and distribution of a single trace dose of ²⁰³HgCl₂ in rats was investigated. The copper-supplemented diet depressed the carcass retention and organ content of mercury-203 given intragastrically. Alterations were accompanied with disturbances in the organ mercury-203 contents. Moreover, the supplements of copper also affected the...
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