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Investigations were conducted on the strongly infested by microbes embryos isolated from seed stratified in non-sterile conditions or from tree buds. The results showed, that PPM (Plant Preservative Mixture, produced by Plant Cell Technology, Inc.) was very useful for increasing the percentage of sterile cultures disinfected in a 5% solution of NaOCl. The best way of applying PPM turned out to be...
Ripe, stratified beechnuts, turned out to be almost impossible to sterilize employing the commonly used sterilizers. The recommended way of sterilizing beech nuts is to soak (for 5 minutes, in a 2% solution of NaOCl) the embryos with parts of cotyledons, isolated from nuts partly sterilized with an 15% solution of NaOCl for 15 minutes. The chloraminę (concentration 4%, time of soaking 2 minutes) provided...
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