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W describe an effective systematic approach to genetic mapping of cDNA clones, including those obtained from EST sequencing. The EST of interest is first partially sequenced from the 3'-end. PCR primers which bracket the 3'-UTR segment of the cDNA are designed. The corresponding gene segment is amplified from the parents of the mapping population, using primers equipped with 3'- and 5'-extensions...
Sprawdzono przydatność metody PCR do oceny dystansu genetycznego linii żyta (Secale cereale L) oraz genetycznego wyrównania dwóch linii. W porównaniu ze starterami o sekwencji przypadkowej (RAPD) startery ukierunkowane na sekwencje styku intron-egzon generują bardziej złożone i polimorficzne obrazy powielonych fragmentów DNA. Obrazy powielenia przy użyciu niektórych starterów 18-nukleotydowych były...
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