The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Protoplasts were isolated from mesophyll leaf tissue of in-vitro-grown 3-4-week-old seedlings of two wild tomato species L. glandulosum C.H. Mull. PI 731163 and L. peruvianum (L.) Mill. PI 128650, and from F4 plants of the Stevens x Rodade hybrid of L. esculentum Mill. For protoplasts isolation from both wild species a mixture of 0.3% cellulase + 0.03% macerozyme + 0.01% MES and 7.0% sorbitol was...
High yields of viable protoplasts were obtained from the mesophyll leaf tissue of young seedlings of Lycopersicon glandulosum grown in vitro at 80 µmol s-1m-2 irradiance and 23°-24°C. The isolated protoplasts were cultured first on a modified semiliquid medium of Tan et al. (1987 b), on which they formed microcalli, and afterwards on a greening medium consisting of MS micro/macro nutrients, Nitsch...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.