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Haploidisation is a biotechnological method used to obtain plants with improved traits that are of use to humans. Lettuce (Lactuca sativa L.), a well-known and popular leafy vegetable, is consumed worldwide. Its haploid form would provide a good basis for producing a pure line of plants (doubled haploids) allowing new varieties to be regenerated. The main aim of this work was to develop an effective...
The factors regulating callus proliferation and bud regeneration from stigma tissues are not sufficiently understood. To study the regenerative capacity of pistil elements, pistil of broccoli was cultured under simple culture conditions. Stigmas with style from broccoli pistils undergo somatic embryogenesis on Murashige and Skoog basal medium. Callus initiation occurred on basal medium supplemented...
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