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Polypodium vulgare L. rhizome cells tolerate water deficit stress to different degrees. This study examined the extent of ultrastructural changes in the endodermis and stelar elements in response to mannitol dehydration treatment. Cytological observations showed that the rhizomes possess structural adaptations to withstand drying by maintaining water inside the stele or activating mechanisms that...
The factors regulating callus proliferation and bud regeneration from stigma tissues are not sufficiently understood. To study the regenerative capacity of pistil elements, pistil of broccoli was cultured under simple culture conditions. Stigmas with style from broccoli pistils undergo somatic embryogenesis on Murashige and Skoog basal medium. Callus initiation occurred on basal medium supplemented...
Placentae of Melandrium album excised from ovaries of unopened flowers were pollinated in vitro with pollen grains of Lychnis coronaria. Pollen grains germinated, and pollen tubes were found entering the micropyles 18 h after pollination. Double fertilization occurred. Immature and mature hybrid embryos at different stages of development were analyzed up to the third week of culture. Twenty-five days...
An efficient protocol for storage of Polypodium vulgare rhizome shoot tips was developed. The best result was observed when the rhizomes were pretreated with 2 mg l-1 abscisic acid/24 h and dehydrated 10 h in hypertonic solution of 20% mannitol before shoot tip isolation. Those explants were encapsulated and stored for 2 months. Plantlets of good quality regenerated after the capsules were transferred...
One-year old shoots of Rhododendron ‘Cunningham’s White’ and R. ‘Nova Zembla’ from which cuttings were obtained. The cuttings consisted of epidermis, periderm, cortex, sclerenchyma, phloem, cambium, xylem and pith. Adventitious roots of cuttings of that taxa were developed throughout basal burls or directly in cambium region.
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