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Two molecular biology methods were used to differentiate Salmonella enterica 1,4,[5],12:i:- strains: “Salmonella Check&Trace microarray” (CT) and multiplex PCR (mPCR). For 92 strains in CT result “Salmonella 1,4,[5],12:i:-” were obtained. Those strains were confirmed in mPCR as monophasic fljB-lack Salmonella Typhimurium. For 17 strains, which in CT assay were recognized as Salmonella Typhimurium,...
Serotyping is a traditional method of Salmonella typing that makes it possible to classify these bacteria by the White-Kauffmann-Le Minor scheme (formerly known as Kauffmann-White scheme) on the basis of variation in the Salmonella somatic (O) and flagellar (H) antigens. It is still the primary method by which Salmonella are identified immediately after the initial isolation of these microorganisms...
Three polypeptide fragments of Hepatitis C virus protein (130-140)C, (133-142)C and (1406-1415)NS3 were synthesized using the solid-phase method. The immunogenicity of the peptides was tested on rabbits. All the peptides studied revealed homoral and cell response.
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