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Previous reports showed that the root extracts of Stellera Chamaejasme L had acaricidal activity against Tetranychus vienneusis Zacher. However, the acaricidal mechanism of the root extracts from Stellera Chamaejasme L against Tetranychus vienneusisis not clear. Here, the effects of the root extracts from Stellera Chamaejasme L. on the activities of three enzymes of mites: protease, glutathiona s-transferase...
Chloroethylnitrosoureas (CENUs) were an important family of anticancer agents in the clinical treatment of human malignancies. Several lines of evidences indicated that their cytotoxicity was related to the interstrand crosslink of DNA. In this study, a method for the determination of DNA crosslink was established using real-time fluorescence quantitative PCR. The DNA crosslink ratio induced by two...
The extract of the K. scoparia had the acardial activity against the T. viennensis. Here the female adult mites were treated by the KFCE-2 extract of K. scoparia, the effective ingredient of the extract of K. scoparia. The toxicosis symptoms of T. viennensis in response to the the KFCE-2 extract of K. scoparia were observed. After treatment, the mites went through four phases: the motionless phase...
Most plants could produce bioactive substances including alkaloids, flavonoids, terpenes and relative materials, which are poisonous or have antifeedant effect to herbivorous animals, including some insects. Some well-known pesticides originated from plant bioactive substances have been successfully identified and used, for example, the azadirachtin from neem, nicotine from tobacco, rotenone and deguelin...
Our previous studies showed that methyl palmitate had the acaricidal activity to T. viennensis. However, the mechanism of action of methyl palmitate on T.viennensis is unknown. Here, the toxicosis symptoms of T.viennensis exposed to methyl palmitate were studied to preliminarily uncover the acaricidal mechanism of methyl palmitate. Methyl palmitate caused dose-dependent mortality of T.viennensis...
Methyl palmitate, a kind of fatty acid methyl ester, has been found in many plants. Many biological functions of methyl palmitate have been found. Recently we isolated the methyl palmitate from the green walnuts husks and found it has acaricidal activity that had not been reported before. Here, the acaricidal activity of methyl palmitate, industrial methyl palmitate and the mixtures of methyl palmitate...
Methyl palmitate has showed acaricidal activity to Tetranychus cinnabarinus in our previous studies. However, the acaricidal mechanism of methyl palmitate to T. cinnabarinus is unknown. It was presumed that methyl palmitate may play its acaricidal activity to T. cinnabarinus by interfering the biological function of muscarinic receptors. In our studies, the most important muscarinic receptor subtypes...
According to the results of quantitative pattern recognition for 153 N-nitroso compounds (NNCs) based on Di-region theory, it is put forward that the esters formed from the metabolism of NNCs on beta- or gamma-position could be alkylating agents of DNA bases with the anchimeric assistance of the N-nitroso group. This viewpoint, combined with the conception of alpha-metabolism activation, can explain...
N-phosphorylamino acids can react to form esters, peptides, phosphoryl ester-exchanged products and phosphoryl group migration products under mild conditions which are very important in researches on the origin of life. It has been proposed that the penta-coordinate phosphorous compounds might be the common intermediates of these reactions, which are the key factor to determine the activities. In...
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