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Genetic engineering can be used to give a protein properties that are advantageous for downstream processing. Many heterologous proteins are degraded at high rates by proteases. Depending on which type of proteolytic degradation is encountered the strategy may be different: induction of inclusion bodies, change of the amino acid sequence in the sensitive site of the product, or protection by fusion...
A method to quantify the impact of proteolysis on accumulation of recombinant proteins in E. coli is described. A much smaller intracellular concentration of staphylococcal protein A (SpA) (14.7 mg . g -1 ) compared to the fusion protein SpA-βgalactosidase (138 mg . g -1 ) is explained by a very high proteolysis rate constant of SpA. The SpA synthesis rate reached a maximum...
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