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Gluten-induced aggregation of K562 cells represents an in vitro model reproducing the early steps occurring in the small bowel of celiac patients exposed to gliadin. Despite the clear involvement of TG2 in the activation of the antigen-presenting cells, it is not yet clear in which compartment it occurs. Herein we study the calcium-dependent aggregation of these cells, using either cell-permeable...
The presence of contaminants in indoor environments can affect the health of occupants and expose them to several kind of risks (respiratory diseases, allergies, skin/eyes/throat irritation, etc.). During winter season, in addition, concentration of chemicals indoor increases in new or refurbished buildings due to limited ventilation and energy-efficient insulation (windows, doors, walls, etc.) with...
The effect of the average pressure and temperature of the column on the adsorption equilibrium of insulin variants on a C 8 bonded silica was studied in isocratic reversed-phase HPLC. Analytical injections of samples of four different insulins (bovine, porcine, Lys-Pro and human recombinant) were carried out at constant flow-rate but under increased average pressure. The temperature dependence...
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