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Electrospinning is an effective procedure for fabricating submicron to nanoscale fibers from synthetic polymer as well as natural proteins. We successfully electrospun regenerated silk protein from cocoons of Bombyx mori to produce random as well as aligned fibers with diameter less than 100nm. The fibers were characterized using field emission environmental scanning electron microscope (ESEM), Fourier...
Computer-aided tissue-engineering approach was used to develop a novel precision extrusion deposition (PED) process to directly fabricate Polycaprolactone (PCL) and composite PCL/hydroxyapatite (PCL–HA) tissue scaffolds. The process optimization was carried out to fabricate both PCL and PCL–HA (25% concentration by weight of HA) with a controlled pore size and internal pore structure of the 0°/90°...
Regenerated Bombyx mori silk fibroin in formic acid was electrospun and the morphological, chemical and mechanical properties of these nanofibers were examined by field emission environmental scanning electron microscopy (FESEM), Raman spectroscopy (RS), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and tensile testing. FESEM indicated that the average fiber diameter was...
Collagen II fibrils are a critical structural component of the extracellular matrix of cartilage providing the tissue with its unique biomechanical properties. The self-assembly of collagen molecules into fibrils is a spontaneous process that depends on site-specific binding between specific domains belonging to interacting molecules. These interactions can be altered by mutations in the COL2A1 gene...
The response surface methodology was used to model and optimize the electrospinning parameters for the spinning of regenerated nanoscale silk fibers from domestic silkworm, Bombyx mori. Electric field and silk concentrations were chosen as variables to control fiber diameter at different spinning distances. Fiber diameter was correlated to these variables by using a second order polynomial function...
We studied the effect of electrospinning parameters on the morphology and fiber diameter of regenerated silk from Bombyx mori. Effects of electric field and tip-to-collection plate distances of various silk concentrations in formic acid on fiber uniformity, morphology and diameter were measured. Statistical analysis showed that the silk concentration was the most important parameter in producing uniform...
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