Search results for: Robert A. Goldbeck

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article

Crowd Control: Effects of Macromolecular Crowding on Early Events in Protein Folding

Eefei Chen, Vanessa Feng, Zachary Fong, Brian Rastian, more

Biophysical Journal > 2014 > 106 > 2 Supplement 1 > 468a

article

Nanosecond time-resolved polarization spectroscopies: Tools for probing protein reaction mechanisms

Eefei Chen, Robert A. Goldbeck, David S. Kliger

Methods > 2010 > 52 > 1 > 3-11

Polarization methods, introduced in the 1800s, offered one of the earliest ways to examine protein structure. Since then, many other structure-sensitive probes have been developed, but circular dichroism (CD) remains a powerful technique because of its versatility and the specificity of protein structural information that can be explored. With improvements in time resolution, from millisecond to picosecond...

article

The Effect of Distal Heme Pocket Mutations on the Water Accessible Areas in Myoglobin

Benjamin C. Rodriguez, Robert A. Goldbeck, Raymond M. Esquerra, Rosa L. Nguyen, more

Biophysical Journal > 2010 > 98 > 3 Supplement 1 > 640a

article

The Effect of Non-Coordinated Water in the Heme Pocket on the Ligand Binding Dynamics of Heme Proteins

Rosa L. Nguyen, Benjamin W. Lintner, Ignacio L. Pena, Pooncharas Tipgunlakant, more

Biophysical Journal > 2010 > 98 > 3 Supplement 1 > 639a-640a

article

Optical Detection of Disordered Water within a Protein Cavity

Robert A. Goldbeck, Marlisa L. Pillsbury, Russell A. Jensen, Juan L. Mendoza, more

Journal of the American Chemical Society > 2009 > 131 > 34 > 12265 - 12272

article

Measurement of the Heme Affinity for Yeast Dap1p, and Its Importance in Cellular Function ^†

Alisha M. Thompson, Amit R. Reddi, Xiaoli Shi, Robert A. Goldbeck, more

Biochemistry > 2007 > 46 > 50 > 14629 - 14637

article

Non-Native Heme−Histidine Ligation Promotes Microsecond Time Scale Secondary Structure Formation in Reduced Horse Heart Cytochrome c ^†

Eefei Chen, Christina J. Abel, Robert A. Goldbeck, David S. Kliger

Biochemistry > 2007 > 46 > 43 > 12463 - 12472

article

Conformational Equilibration Time of Unfolded Protein Chains and the Folding Speed Limit ^†

Christina J. Abel, Robert A. Goldbeck, Ramil F. Latypov, Heinrich Roder, more

Biochemistry > 2007 > 46 > 13 > 4090 - 4099

article

Spectroscopic and Biochemical Characterization of Heme Binding to Yeast Dap1p and Mouse PGRMC1p ^†

Kaushik Ghosh, Alisha M. Thompson, Robert A. Goldbeck, Xiaoli Shi, more

Biochemistry > 2005 > 44 > 50 > 16729 - 16736

article

The Molecular Code for Hemoglobin Allostery Revealed by Linking the Thermodynamics and Kinetics of Quaternary Structural Change. 1. Microstate Linear Free Energy Relations ^†

Robert A. Goldbeck, Raymond M. Esquerra, Jo M. Holt, Gary K. Ackers, more

Biochemistry > 2004 > 43 > 38 > 12048 - 12064

article

The Molecular Code for Hemoglobin Allostery Revealed by Linking the Thermodynamics and Kinetics of Quaternary Structural Change. 2. Cooperative Free Energies of (α _FeCO β _Fe ) ₂ and (α _Fe β _FeCO ) ₂ T-State Tetramers ^†

Robert A. Goldbeck, Raymond M. Esquerra, David S. Kliger, Jo M. Holt, more

Biochemistry > 2004 > 43 > 38 > 12065 - 12080

article

The Earliest Events in Protein Folding: A Structural Requirement for Ultrafast Folding in Cytochrome c

Eefei Chen, Robert A. Goldbeck, David S. Kliger

Journal of the American Chemical Society > 2004 > 126 > 36 > 11175 - 11181

article

Earliest Events in Protein Folding: Submicrosecond Secondary Structure Formation in Reduced Cytochrome c ^† ^, ^‡

Eefei Chen, Robert A. Goldbeck, David S. Kliger

The Journal of Physical Chemistry A > 2003 > 107 > 40 > 8149 - 8155

article

Hydrogen Bonding to Trp β37 Is the First Step in a Compound Pathway for Hemoglobin Allostery

Robert A. Goldbeck, Raymond M. Esquerra, David S. Kliger

Journal of the American Chemical Society > 2002 > 124 > 26 > 7646 - 7647

article

The Effect of Water on the Rate of Conformational Change in Protein Allostery

Robert A. Goldbeck, Sarah J. Paquette, David S. Kliger

Biophysical Journal > 2001 > 81 > 5 > 2919-2934

The influence of solvation on the rate of quaternary structural change is investigated in human hemoglobin, an allosteric protein in which reduced water activity destabilizes the R state relative to T. Nanosecond absorption spectroscopy of the heme Soret band was used to monitor protein relaxation after photodissociation of aqueous HbCO complex under osmotic stress induced by the nonbinding cosolute...

article

Absolute Kinetic Characterization of 17-β-Estradiol as a Radical-Scavenging, Antioxidant Synergist

John S. Winterle, Theodore Mill, Tennile Harris, Robert A. Goldbeck

Archives of Biochemistry and Biophysics > 2001 > 392 > 2 > 233-244

We directly measured the absolute reactivity of 17-β-estradiol (E₂) and several phenolic model compounds for E₂ toward t-butoxy radical (t-BuO·) by nanosecond time-resolved optical spectroscopy. Compared to other phenols, E₂ is a moderate, but not strong deactivator of oxyradicals. The absolute bimolecular rate constant for H-atom transfer from E₂ to t-BuO· is 1.3 ± 0.3 × 10⁹ M⁻¹ s⁻¹ (23°C, benzene)...

article

Multiple Geminate Ligand Recombinations in Human Hemoglobin

Raymond M. Esquerra, Robert A. Goldbeck, Stephen H. Reaney, Abigail M. Batchelder, more

Biophysical Journal > 2000 > 78 > 6 > 3227-3239

The geminate ligand recombination reactions of photolyzed carbonmonoxyhemoglobin were studied in a nanosecond double-excitation-pulse time-resolved absorption experiment. The second laser pulse, delayed by intervals as long as 400ns after the first, provided a measure of the geminate kinetics by rephotolyzing ligands that have recombined during the delay time. The peak-to-trough magnitude of the Soret...

article

Evidence for Heme–Heme Excitonic Coupling in the Soret Circular Dichroism of Hemoglobin

Robert A. Goldbeck, Laura Sagle, Daniel B. Kim-Shapiro, Veronica Flores, more

Biochemical and Biophysical Research Communications > 1997 > 235 > 3 > 610-614

In order to study interdimer heme–heme electronic interactions in human hemoglobin, the Soret circular dichroism spectrum of the carboxy adduct is measured as a function of protein concentration, the spectrum at the highest concentration representing primarily that of α ₂ β ₂ tetramers (93%) and the lowest concentration representing primarily αβ dimers (68%). The tetramer–dimer difference...

article

A Study of the Mechanisms of Slow Religation to Sickle Cell Hemoglobin Polymers Following Laser Photolysis

Daniel B. Shapiro, Raymond M. Esquerra, Robert A. Goldbeck, Samir K. Ballas, more

Journal of Molecular Biology > 1996 > 259 > 5 > 947-956

Time-resolved linear dichroism (TRLD) measurements are conducted on gels of sickle cell hemoglobin following laser photolysis of the carbonyl adduct to monitor religation kinetics to hemoglobin S polymers. The return of the polymer phase to its equilibrium ligation state has been found to be about 1000 times slower than that of the solution phase hemoglobin tetramers. Several mechanisms describing...

article

Biexponential fluorescence decay of diphenylbutadiene rotational conformers after extreme red edge excitation

Søren Møller, W. Atom Yee, Robert A. Goldbeck, Stacie E. Wallace-Williams, more

Chemical Physics Letters > 1995 > 243 > 5-6 > 579-585

After excitation at the extreme red edge (358 nm) of the S ₁ ← S ₀ absorption band, the ambient temperature fluorescence decay of all-trans-1,4-diphenyl-1,3-butadiene (DPB) in cyclohexane is biexponential, indicating the presence of two distinct molecular species with substantially different excited-state lifetimes of 0.6 and 9 ns. The enthalpic barrier to nonradiative decay of 3...

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17-Β-ESTRADIOL (1)
ALZHEIMER'S DISEASE (1)
ANTIOXIDANT SYNERGISM (1)
ASCORBATE (1)
ATHEROSCLEROSIS (1)
CIRCULAR DICHROISM (1)
ESTROGEN (1)
HYDROGEN ATOM TRANSFER (1)
MAGNETIC CIRCULAR DICHROISM (1)
OPTICAL ROTATORY DISPERSION (1)
PEROXY RADICAL (1)
PHENOXY RADICAL (1)
POLARIZATION SPECTROSCOPY (1)
PROTEIN FOLDING (1)
SICKLE CELL HEMOGLOBIN; TIME RESOLVED LINEAR DICHROISM; POLYMERIZATION; KINETICS; LASER PHOTOLYSIS (1)
T-BUTOXY RADICAL (1)
TEMPERATURE-JUMP (1)
TIME-RESOLVED (1)
Α-TOCOPHEROL (1)
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Search results for: Robert A. Goldbeck

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