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The realization of a novel microfluidic device for inducing cell rotation is reported. It exploits the hydrodynamic forces acting on cells moving in a flow characterized by a parabolic velocity profile. The device operation was tested on yeast cells, since they have an asymmetrical shape due to a bud formation during the duplication phase. Image processing analysis of the experimental data allowed...
A novel parallel-channel microfluidic device exploiting cross-filtering to perform hydrodynamic focusing has been designed and successfully tested with Leukemia cells. The proposed approach allows to overcome the main limits till now presented by previous devices, and represents a step forward the realization of an on-chip integrated cytofluorimeter.
Flow focusing is a fundamental prior step in order to sort, analyze and detect particles or cells. In traditional flow-cytometry flow focusing is achieved by hydrodynamic focusing. An additional sheath fluid is used in order to confine the sample one. The main drawback of the method is the need of at least two inlets, one for the sheath and one for the sample fluid. This adds a big limitation: it...
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