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A microfluidic device based on hydrodynamic fractionation by size was applied for enrichment of muscle precursor cells obtained from heterogeneous primary cells of muscle tissues. In this paper, the relationship between cell size and quality of regenerative muscles in vitro was focused on. Fractionation of primary cells from rat limbs was demonstrated using a microfluidic device based on hydrodynamics...
This paper reports a novel soft robot consisting only of cardiomyocyte gel and the its chemical control. It has a jellyfish shape and can move on the bottom of a petri dish using cardiomyocyte pulsation. The pulsation, acceleration and on-off switching of the jellyfish-shaped micro robot could be controlled by chemical stimulation. This study will develop a new application of muscle cell actuated...
We have fabricated a muscle-powered micro gripper controlled by electromyogram (EMG) signals as one step toward development of a teleoperated micromanipulator. We utilized human muscles EMG signals as the master control and tissue-engineered skeletal muscles as the slave actuator. This muscle-to-muscle interface for master-slave control is useful for improvement of teleoperated micromanipulation....
This paper reports the first demonstration of a light-regulated bio-micro-actuator powered by a transgenic Drosophila melanogaster DV tissue (insect heart muscle). We expressed a mutant form of blue light-sensitive cation channel, channelrhodopsin-2, into the cell membrane of the DV tissue. The DV tissue was assembled into a micro-structure made of poly-dimethylsiloxane (PDMS). The light-sensitive...
Here we propose a room temperature operable muscle powered bioactuator by dorsal vessel (tubular insect heart) cells. Insect cells are generally robust over a range of culture conditions compared to mammalian cells. In this study, we conducted two experiments to show the potential of the insect cell as an actuator. First, beating frequency and beating force of dorsal vessels as both tissues and cells...
Electrical stimulation was applied to drive rat skeletal muscle cells. Then this driving force was utilized to fabricate a diaphragm type micropump. First, a skeletal muscle cell sheet, which consisted of skeletal muscle cells on a PDMS sheet, was fabricated to extract the drive force from the cells. These cells are known to align along a micro pattern. We took advantage of this principle and patterned...
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