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Hypoxia‐inducible factors (HIFs) play a critical role in inflammatory properties of myeloid‐derived cells. The effect of HIFs on myeloid‐derived cell functions in organ transplantation remains unknown, however. We transplanted hearts into transgenic mice with myeloid cell‐targeted deletions of HIF‐1α or its negative regulator von Hippel–Lindau (VHL) to investigate the effects of HIF‐1α inactivation...
Heart transplant gene therapy requires vectors with long‐lasting gene expression, high cardiotropism, and minimal pathological effects. Here, we examined transduction properties of ex vivo intracoronary delivery of adeno‐associated virus (AAV) serotype 2, 8, and 9 in rat syngenic and allogenic heart transplants. Adult Dark Agouti (DA) rat hearts were intracoronarily perfused ex vivo with AAV2, AAV8,...
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