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Poly(N‐isopropylacrylamide‐co‐acrylamide) (PNIAM‐co‐AM) grafted onto tissue culture polystyrene surfaces (TCPSs) with different thicknesses and grafting densities were prepared by varying the spin‐coating speed and UV irradiation time for use in cell sheet engineering. The thickness of the PNIAM grafted surface, less than 30 nm, was believed to influence protein adhesion and cell attachment. However,...
Wound closure is a crucial step in the treatment of burns, often performed using autologous skin graft. Unfortunately, in extensive burn injuries, only limited area of normal skin can be used to make a graft which is not enough to cover the entire wound surface. To overcome the shortage of the skin donor site, a skin graft constructed from a sheet of co-cultured human dermal fibroblast and keratinocyte...
In this study, a novel temperature-responsive poly(N-isopropylacrylamide)-co-acrylamide was used to prepare a chondrocyte cell sheet. Chondrocytes were isolated from human articular cartilage and plated on the copolymer film grafted tissue culture plates. The cell attachment on the copolymer film was shown to be similar to that of the ungrafted surface. To harvest a cell sheet, the incubation temperature...
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