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YrdD, a homolog of the C-terminal zinc-binding region of Escherichia coli topoisomerase I, is highly conserved among proteobacteria and enterobacteria. However, the function of YrdD remains elusive. Here we report that YrdD purified from E. coli cells grown in LB media contains both zinc and iron. Supplement of exogenous zinc in the medium abolishes the iron binding of YrdD in E. coli cells, indicating...
Key messageBjYSL7encodes a plasma-localized metal–NA transporter and has transport Fe(II)–NA complexes activity. BjYSL7 is involved in the transport of Cd and Ni from roots to shoots. Abstract Heavy metal transporters play a key role in regulating metal accumulation and transport in plants. In this study, we isolated a novel member of the yellow stripe-like (YSL) gene family BjYSL7 from the...
The purpose of this paper was to know the elderly diet and nutritional status of elderly apartments in Shandong province, then give some suggestions for improvement. The method of investigation was a continuous 3-day bookkeeping meanwhile conbing the retrospection, which lasts 2 months in 45 apartments of six regions (effective 38), Shandong province. The results is as follows: the composition of...
Escherichia coli topoisomerase I (TopA) cleaves and rejoins one strand of double-stranded DNA to relax the negatively supercoiled DNA. Structurally, TopA contains an N-terminal catalytic fragment and a C-terminal zinc-binding region that is required for relaxation of the negatively supercoiled DNA. Here we report that E. coli TopA is an iron and zinc binding protein. The UV–Vis absorption measurements...
The influence of Cu on the dechlorination rate of various chlorinated hydrocarbons including CF, CT, 1,1,2,2-TeCA, 1,1,2,2-TeCA and HCA by Fe0 and Cu/Fe was investigated in batch experiments. All organohalides can be dechlorinated rapidly by Fe or Cu/Fe in aqueous system and the contaminants were degraded via sequential reductive dechlorination to form less chlorinated homologues. The removal of CF,...
Lipid peroxidation in relation to toxicity of detached rice leavescaused by excess iron (FeSO4) was investigated. ExcessFeSO4, which was observed to induce toxicity, enhanced the contentoflipid peroxidation but not the content of H2O2.Superoxidedismutase activity was reduced by excess FeSO4. Ascorbate peroxidaseand glutathione reductase activities were increased by excess FeSO4.Free radical scavengers,...
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