Search results for: Takeshi Katsuda

Items from 1 to 16 out of 16 results

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The yin and yang of pioneer transcription factors: Dual roles in repression and activation

Takeshi Katsuda, Jonathan H. Sussman, Kenneth S. Zaret, Ben Z. Stanger

Pioneer transcription factors, by virtue of their ability to target nucleosomal DNA in silent chromatin, play crucial roles in eliciting cell fate decisions during development and cellular reprogramming. In addition to their well‐established role in chromatin opening to activate gene expression programs, recent studies have demonstrated that pioneer factors have the complementary function of being...

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Dynamic Transcriptional and Epigenetic Changes Drive Cellular Plasticity in the Liver

Allyson J. Merrell, Tao Peng, Jinyang Li, Kathryn Sun, more

Hepatology > 74 > 1 > 444 - 457

Background and Aims Following liver injury, a fraction of hepatocytes adopt features of biliary epithelial cells (BECs) in a process known as biliary reprogramming. The aim of this study was to elucidate the molecular events accompanying this dramatic shift in cellular identity. Approach and Results We applied the techniques of bulk RNA‐sequencing (RNA‐seq), single‐cell RNA‐seq, and assay for transposase‐accessible...

article

Cover Image, Volume 118, Number 7, July 2021

Yu Huang, Yusuke Sakai, Takanobu Hara, Takeshi Katsuda, more

Biotechnology and Bioengineering > 118 > 7 > i - i

The cover image is based on the Article Bioengineering of a CLiP‐derived tubular biliary‐duct‐like structure for bile transport in vitro by Yu Huang et al., https://doi.org/10.1002/bit.27773.

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Bioengineering of a CLiP‐derived tubular biliary‐duct‐like structure for bile transport in vitro

Yu Huang, Yusuke Sakai, Takanobu Hara, Takeshi Katsuda, more

Biotechnology and Bioengineering > 118 > 7 > 2572 - 2584

The integration of a bile drainage structure into engineered liver tissues is an important issue in the advancement of liver regenerative medicine. Primary biliary cells, which play a vital role in bile metabolite accumulation, are challenging to obtain in vitro because of their low density in the liver. In contrast, large amounts of purified hepatocytes can be easily acquired from rodents. The in...

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Long‐term maintenance of functional primary human hepatocytes using small molecules

Takeshi Katsuda, Masaki Kawamata, Ayako Inoue, Tomoko Yamaguchi, more

FEBS Letters > 594 > 1 > 114 - 125

The immediate deterioration of primary human hepatocytes (PHHs) during culture limits their utility in drug discovery studies. Here, we report that a cocktail of four small molecule signaling inhibitors, termed YPAC, is useful for maintaining various hepatic functions of PHHs, including albumin and urea productivity, glycogen storage, and cytochrome P450 (CYP) expression. Most importantly, we found...

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Generation of functional human hepatocytes in vitro: current status and future prospects

Tomoko Yamaguchi, Juntaro Matsuzaki, Takeshi Katsuda, Yoshimasa Saito, more

Inflammation and Regeneration > 2019 > 39 > 1 > 1-9

Liver and hepatocyte transplantation are the only effective therapies for late-stage liver diseases, in which the liver loses its regenerative capacity. However, there is a shortage of donors. As a potential alternative approach, functional hepatocytes were recently generated from various cell sources. Analysis of drug metabolism in the human liver is important for drug development. Consequently,...

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In vitro reconstitution of breast cancer heterogeneity with multipotent cancer stem cells using small molecules

Masaki Kawamata, Takeshi Katsuda, Yasuhiro Yamada, Takahiro Ochiya

Biochemical and Biophysical Research Communications > 2017 > 482 > 4 > 750-757

A small fraction of tumor cells are thought to possess the potential for both multiple-lineage differentiation and self-renewal, which underlies the cancer stem cell hypothesis. However, the differentiation mechanisms of these cells have not been elucidated due to a lack of appropriate culture methods. Here, we established a culture condition for maintaining multipotent tumor cells from rat breast...

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Conversion of Terminally Committed Hepatocytes to Culturable Bipotent Progenitor Cells with Regenerative Capacity

Takeshi Katsuda, Masaki Kawamata, Keitaro Hagiwara, Ryou-u Takahashi, more

Cell Stem Cell > 2017 > 20 > 1 > 41-55

A challenge for advancing approaches to liver regeneration is loss of functional differentiation capacity when hepatocyte progenitors are maintained in culture. Recent lineage-tracing studies have shown that mature hepatocytes (MHs) convert to an immature state during chronic liver injury, and we investigated whether this conversion could be recapitulated in vitro and whether such converted cells...

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Commitment of Annexin A2 in recruitment of microRNAs into extracellular vesicles

Keitaro Hagiwara, Takeshi Katsuda, Luc Gailhouste, Nobuyoshi Kosaka, more

FEBS Letters > 2015 > 589 > 24(PB) > 4071-4078

Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray analysis revealed that ANXA2 regulated the loading of miRNAs into EVs in a sequence...

article

Commitment of Annexin A2 in recruitment of microRNAs into extracellular vesicles

Keitaro Hagiwara, Takeshi Katsuda, Luc Gailhouste, Nobuyoshi Kosaka, more

FEBS Letters > 589 > 24PartB > 4071 - 4078

article

Micromanaging of tumor metastasis by extracellular vesicles

Naoomi Tominaga, Takeshi Katsuda, Takahiro Ochiya

Seminars in Cell and Developmental Biology > 2015 > 40 > Complete > 52-59

Extracellular vesicles (EVs) are nanometer-sized membranous vesicles that are released by a variety of cell types into the extracellular space. In the past two decades, EVs have emerged as novel mediators of cancer biology. Many reports have demonstrated the contribution of EVs to cancer metastasis. Metastasis is a multistep process that is responsible for the majority of deaths in cancer patients...

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Molecular signatures of mesenchymal stem cell-derived extracellular vesicle-mediated tissue repair

Takeshi Katsuda, Takahiro Ochiya

Stem Cell Research & Therapy > 2015 > 6 > 1 > 1-8

Extracellular vesicles (EVs) play important roles in intercellular communications via their content molecules, and mimic, at least in part, the roles that are played by their originating cells. Consistent with this notion, an increasing number of reports have suggested that EVs derived from mesenchymal stem cells (MSCs), which are therapeutically beneficial to a wide range of diseases, can serve as...

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The roles of extracellular vesicles in cancer biology: Toward the development of novel cancer biomarkers

Takeshi Katsuda, Nobuyoshi Kosaka, Takahiro Ochiya

PROTEOMICS > 14 > 4-5 > 412 - 425

Recent important progress in cancer biology was the identification of the significant roles played by extracellular vesicles (EVs). EVs are secreted by a variety of mammalian cell types and have been revealed to play important roles in intercellular communications. EVs serve as unique communication vehicles in many ways. First, unlike cytokine signaling, EVs enable transportation not only of proteins,...

article

The therapeutic potential of mesenchymal stem cell‐derived extracellular vesicles

Takeshi Katsuda, Nobuyoshi Kosaka, Fumitaka Takeshita, Takahiro Ochiya

PROTEOMICS > 13 > 10-11 > 1637 - 1653

Extracellular vesicles (EVs), membrane vesicles that are secreted by a variety of mammalian cell types, have been shown to play an important role in intercellular communication. The contents of EVs, including proteins, microRNAs, and mRNAs, vary according to the cell type that secreted them. Accordingly, researchers have demonstrated that EVs derived from various cell types play different roles in...

article

Hypoxia efficiently induces differentiation of mouse embryonic stem cells into endodermal and hepatic progenitor cells

Takeshi Katsuda, Takumi Teratani, Mohammad Mahfuz Chowdhury, Takahiro Ochiya, more

Biochemical Engineering Journal > 2013 > 74 > Complete > 95-101

Although ES cells have potential impact on cell-based therapy for liver diseases, their efficient differentiation into functional hepatocytes remains difficult. One possible approach to this issue is to control the culture environment to recapitulate the in vivo liver development. Given that the embryo is exposed to hypoxic condition in its early stage, hypoxic condition seems suitable for inducing...

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Enhanced effects of secreted soluble factor preserve better pluripotent state of embryonic stem cell culture in a membrane-based compartmentalized micro-bioreactor

Mohammad Mahfuz Chowdhury, Takeshi Katsuda, Kevin Montagne, Hiroshi Kimura, more

Biomedical Microdevices > 2010 > 12 > 6 > 1097-1105

Pluripotent stem cells are under the influence of soluble factors in a diffusion dominant in vivo microenvironment. In order to investigate the effects of secreted soluble factors on embryonic stem cell (ESC) behavior in a diffusion dominant microenvironment, we cultured mouse ESCs (mESCs) in a membrane-based two-chambered micro-bioreactor (MB). To avoid disturbing the cellular environment in the...

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