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The Cladosporium fulvum Avr2 effector is a novel type of cysteine protease inhibitor with eight cysteine residues that are all involved in disulphide bonds. We have produced wild‐type Avr2 protein in Pichia pastoris and determined its disulphide bond pattern. By site‐directed mutagenesis of all eight cysteine residues, we show that three of the four disulphide bonds are required for Avr2 stability...
From a set of Phytophthora infestans cDNA clones randomly selected from a potato-P. infestans interaction cDNA library, three out of 22 appeared to correspond to a gene encoding translation elongation factor 1α. The gene, called tef1, is a single copy gene in P. infestans. During the life cycle of P. infestans, tef1 is expressed in all developmental stages. Alignment and phylogeny analysis based on...
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