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The Streptococcus pneumoniae Rgg144/SHP144 regulator‐peptide quorum sensing (QS) system is critical for nutrient utilization, oxidative stress response, and virulence. Here, we characterized this system by assessing the importance of each residue within the active short hydrophobic peptide (SHP) by alanine‐scanning mutagenesis and testing the resulting peptides for receptor binding and activation...