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In this work, we propose a novel genetic pathway discovery and comparison analysis framework integrating newly generated gene expression microarray data and existing biological pathway information. Starting with the significance analysis of microarray (SAM), a list of differentially expressed genes among groups is obtained. This gene list is then imported to the Ingenuity Pathway Analysis (IPA) to...
In this study, we examined the effects of exposure to electromagnetic pulse(EMP) on the functional integrity of the BBB and, on the expression of tight junction (TJ) protein (occludin, ZO-1) in rats. Animals were whole body exposed or sham exposed to EMP at 200 kV/m for 400 pulses. The permeability of BBB was examined using Evans Blue (EB) as vascular tracer. The expression of the level of TJ proteins...
AIM To discuss the effects of EMP on bonding ability of insulin and itpsilas receptor. METHODS HL-7702[L-02]GNHu6 cells (5times105 ldr mL-1) were divided into 2 groups: anti-insulin-fluorescent labeling group (including cell-irrad + insulin-irrad group, cell-irrad + normal insulin group, normal cell + insulin-irrad group, normal cell + normal insulin group, total 4 sub-groups) and anti-insulin receptor-fluorescent...
To investigate the changes of the mice testes and the tight junction (Tj) relative molecular and to identify the molecular mechanisms of the mice blood-testis-barrier (BTB) permeability increasing after the mice were exposed to electromagnetic pulse (EMP). Observe the changes vimentin of the mice testes by laser scanning confocal technique. After the 200 kVm-1 EMP (the accumulative pulse times was...
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