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A new high‐speed counter‐current chromatography method for semi‐preparative separation and purification of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn was developed by using pH‐gradient elution mode. Diethyl ether was used as the stationary phase of the two‐phase solvent system and Na2HPO4/NaH2PO4 buffer solution with pH values of 7.5 and 7.2 in gradient mode as the mobile phase....