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Skeletonema is a dominant diatom, especially in coastal waters. However, species identification and quantification in the natural environment has not yet been established, as species identification can be nearly impossible with light microscopy. In the present study, we developed primer sets for multiplex and real-time PCR to identify and enumerate the 10 Skeletonema species, and applied this technique...
We investigated shifts in growth rates and cell size reduction–restoration processes in two species of diatoms, Skeletonema japonicum and Skeletonema dohrnii. Growth rates greatly fluctuated from 1.0 to 2.1 per day, even under the same conditions. Rates increased from 1.1 to 2.1 per day with decreasing valve diameter; however, rates quickly decreased to 1.0 per day when cell size reached the threshold...
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