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Sulfate-reducing bioreactors (SRBRs) represent a passive, sustainable, and long-term option for mitigating mining influenced water (MIW) during release. Here we investigate spatial zinc precipitation profiles as influenced by substrate differentiation, inorganic ligand availability (inorganic carbon and sulfide), and microbial community structure in pilot-scale SRBR columns fed with sulfate and zinc-rich...
An improved in situ hybridization approach (Polygold‐FISH) using biotinylated probes targeting multiple locations of the 16 S ribosomal subunit, followed by fluoronanogold‐streptavidin labeling and autometallographic enhancement of nanogold particles was developed as a means of signal amplification of metallo‐labeled cells, without the need for Catalyzed Reporter Deposition (CARD). Bacterial cells...
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