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Polyomavirus BK (BKPyV) T‐antigens (large and small tumor antigens, or Lt‐ag and st‐ag, respectively), control key aspects of viral replication and are able to regulate cell cycle, promoting cell proliferation. However, the structural effects of genetic mutations on T‐antigens are poorly investigated. In this study, 214 sequences of T‐antigens from individuals with different BKPyV infections (16 renal...