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Although the use of hemoglobin-containing food ingredients (HCFIs) in meat and non-meat products is increasing, there is no viable method for monitoring their usage. The purpose of this study was to investigate the potential of a ruminant-hemoglobin-specific monoclonal antibody (mAb) Bb1H9 as the probe for the immunodetection of bovine HCFIs in a wide range of commercially-processed sausages and dietary...
A sandwich enzyme‐linked immunosorbent assay (sELISA) based on 2 monoclonal antibodies (Bb3D6 and Bb6G12) that recognize a 60‐kDa antigenic protein in bovine blood was previously developed for detecting bovine blood in animal feed for the prevention of mad cow disease. This study sought to establish the identity of this 60‐kDa antigenic protein and consequently determine the suitability of the sELISA...
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